Resumen Antecedentes: Los virus se utilizan como herramientas alternativas y complementarias para el tratamiento del cáncer. Los virus oncolíticos exhiben tropismo por tumores, capacidad para intensificar la inmunidad antitumoral y la capacidad para utilizarse en combinación con quimioterapia y radioterapia convencionales. Recientemente, hemos seleccionado algunos aislamientos de rotavirus que están adaptados para infectar y eliminar de manera eficiente líneas de células tumorales. Objetivo: Se ensayaron cinco aislamientos de rotavirus adaptados a células tumorales para determinar su capacidad para infectar la línea celular de adenocarcinoma humano MCF-7. Métodos: Las proteínas asociadas a la membrana de la superficie celular que median la unión de partículas de virus se caracterizaron mediante ELISA, inmunoprecipitación, análisis FACS y bloqueo de anticuerpos. Resultados: Se encontró que las proteínas de choque térmico (HSPs) como Hsp90, Hsp70, Hsp60 y Hsp40 se expresan en la superficie celular formando complejos con la proteína disulfuro isomerasa (PDI), la integrina β3 y la proteína análoga de choque térmico 70 (Hsc70) en microdominios lipídicos (rafts). La interacción de los aislamientos de rotavirus con estas proteínas celulares se confirmó adicionalmente mediante un ensayo de competición y un ensayo de inhibición que incluía las HSP ensayadas. Conclusión: Nuestros hallazgos sugieren que los aislamientos de rotavirus adaptados a las células tumorales estudiados aquí ofrecen una herramienta prometedora para eliminar las células tumorales, lo que estimula más investigaciones sobre este tema, incluidos los modelos animales.

Abstract Background: Viruses are being used as alternative and complementary tools for treating cancers. Oncolytic viruses exhibit tumor tropism, ability to enhance anti-tumor immunity and ability to be used in combination with conventional chemotherapy and radiotherapy. We have recently selected some rotavirus isolates which are adapted to efficiently infect and kill tumor cell lines. Aim: We tested five tumor cell-adapted rotavirus isolates for their ability to infect the human adenocarcinoma cell line MCF-7. Methods: Cell surface membrane-associated proteins mediating virus particle attachment were characterized using ELISA, immunoprecipitation, FACS analysis, and antibody blocking. Results: It was found that heat shock proteins (HSPs) such as Hsp90, Hsp70, Hsp60, and Hsp40 are expressed on the cell surface forming complexes with protein disulfide isomerase (PDI), integrin β3, and heat shock cognate protein 70 (Hsc70) in lipid raft microdomains. Interaction of rotavirus isolates with these cellular proteins was further confirmed by a competition assay and an inhibition assay involving the HSPs tested. Conclusion: Our findings suggest that the tumor cell-adapted rotavirus isolates studied here offer a promising tool for killing tumor cells, thus encouraging further research into this topic, including animal models.

Abstract The industrial by-product of blueberry juice, a source of fibre with antioxidant capacity was used to develop cookies. Once dried and ground the blueberries by-product, its composition and functional properties were analyzed and used to develop fibre enriched cookies. A central composite design was used to optimize the cookie formulation maximizing antioxidant and total polyphenol content varying fibre content (3-9 g/100 g), baking temperature (160-180 °C) and dough thickness (0.5-1.0 cm). Antioxidant capacity and total polyphenol content showed both a similar behaviour, increasing when fibre content increases. The increasing of dough thickness and baking temperature decreases the antioxidant capacity and total polyphenol content. Cookie with 9% fibre, 180 °C baking temperature and 0.50 cm dough thickness and cookie with 9% of fibre, 170°C baking temperature and 0.75 cm dough thickness showed maximum antioxidant capacity and polyphenol content with no significant differences between them; both cookies can be labelled using a fibre claim.

La Infección del Tracto Urinario (ITU) es una de las patologías infecciosas más frecuentes en pediatría. Como factores de riesgo más relevantes se encuentran el reflujo vesicoureteral y las malformaciones urogenitales. El estudio con imágenes es imprescindible para detectar posibles anomalías del tracto urinario, demostrando la ecografía la mejor correlación costo-beneficio. Objetivo: Describir las características y resultados de las ecografías renales y vesicales de pacientes hospitalizados por ITU en el Hospital San Martín de Quillota (HSMQ) en el año 2014. Material y métodos: Estudio descriptivo; estudio de casos, se incluyó a niños hospitalizados en el servicio de pediatría del HSMQ durante el año 2014, ingresados con diagnóstico de ITU. Resultados: Ingresos por ITU: 99 pacientes, excluyéndose 27 casos con un número total de 72 pacientes; de estos, 71 % fueron de sexo femenino, 76% lactantes. Del total de ecografías, 55% resultaron normales, 3 1% compatibles con Pielonefritis Aguda (PNA) y 14% con alteración anatómica, siendo lo más frecuente el doble sistema pielocalicial. Discusión: Hubo similitud entre la información entregada por la literatura de un 12 a 15% de ecografías alteradas y un 14% en este estudio.

The Urinary Tract Infection (UTI) is one of the most common infectious diseases in children. Vesicouretral reflux and urogenital malformations are amongst the most significant risk factors. The imagenological studies are essential to detect possible anatomical or functional urological abnormalities. Within them, renal ultrasound (US) has the best cost-benefit relation. Objective: Describe the characteristics and results of renal and vesical ultrasound done on patients hospitalized in Hospital San Miguel de Quillota (HSMQ) during 2014, diagnosed with UTI. Material and methods: Descriptive study, cases study includes hospitalized children under the diagnose of UTI in the Pediatric Service of HSMQ during 2014. Results: 99 patients entered with the diagnose of UTI, 27 cases were excluded,with 72 patient which met the inclusion criteria:71% of them were female and 76% were infants. From the total of US, 55% were normal, 3 1% showed acute pyelonephritis (APN) and 14% anatomical abnormalities, where duplicated collecting system was the highest frequency condition. Discussion: There were similar results in literature that showed 12-15% of altered ultrasound compared to 14% obtained in this study.

This study evaluated the expression of defense compounds by the secondary metabolism of Eucalyptus globulus plants subjected to attacks by the insect Ctenarytaina eucalypti (blue gum psyllid) and abiotic mechanical damage using gas chromatography with flame ionization detection (GC-FID) and mass detection (MS). The results show that both biological and mechanical attacks activated defense responses in the plants. We identified 34 compounds in the leaves of the treated plants. In the case of the plants damaged by C. eucalypti, four of the compounds differed from those of the control: three oxygenated monoterpenes (borneol, exo-2-hydroxy cineole and thymol) and one quinone (6-acethyl-flaviolin). In the case of the mechanical damage, five compounds differed from the control: three sesquiterpenes (alloaromadendrene, eremophilene, and caryophyllene-oxide), borneol, and 6-acethyl-flaviolin. According to the results, different compounds are biosynthesized when faced with biotic and abiotic inductions, indicating a certain level of specificity in the response of the plants to different types of damage.